POLR1A Polyclonal antibody

POLR1A Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Publications

2

Reviews (1)

Cat no : 20595-1-AP

Synonyms

A190, POLR1A, RNA polymerase I subunit A1, RPA1, RPA194, RPO1 4

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Validation Data Gallery

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  WB analysis of HeLa using 20595-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20595-1-AP (POLR1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 20595-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20595-1-AP (POLR1A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 20595-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20595-1-AP (POLR1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 20595-1-AP

  IP result of anti-POLR1A (IP:20595-1-AP, 4ug; Detection:20595-1-AP 1:300) with HeLa cells lysate 2400ug.

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, HepG2 cells
• Positive IP detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IP: See 1 publications below

Product Information

20595-1-AP targets POLR1A in WB, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: POLR1A fusion protein Ag14543
• Full Name: polymerase (RNA) I polypeptide A, 194kDa
• Calculated Molecular Weight: 1720 aa, 195 kDa
• Observed Molecular Weight: 190-195 kDa
• GenBank Accession Number: BC017115
• Gene Symbol: POLR1A
• Gene ID (NCBI): 25885
• RRID: AB_10949227
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

POLR1A, DNA-directed RNA polymerase I subunit RPA1, is a DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA. RNA polymerase I (Pol I) located in the nucleolus and transcribes class I genes, which code for large ribosomal RNA. Different subunits of the Pol I transcription are targets of various physiological stimuli, which suggests that multiple signaling pathways are involved in carrying out Pol I ranscription. Together with the second largest subunit, it is the catalytic core component of RNA polymerase I that synthesizes ribosomal RNA precursors.

Publications

Species	Application	Title
		Cell; RNA polymerase III is required for the repair of DNA double-strand breaks by homologous recombination.; Authors - Sijie Liu
human	IP	Cancer Sci; TCOF1 coordinates oncogenic activation and rRNA production and promotes tumorigenesis in HCC.; Authors - Chaoshen Wu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Anita (Verified Customer) (12-22-2020)

HeLa cell protein extract (5micrograms)

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HeLa cells

POLR1A Antibody Western Blot validation (1:1000 dilution) in HeLa cells (Cat no:20595-1-AP)