POLM Polyclonal antibody

POLM Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14873-1-AP

Synonyms

DNA polymerase mu, Pol Mu, POLM, polymerase (DNA directed), mu, Tdt N

---

Validation Data Gallery

• 

  WB analysis of Jurkat using 14873-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14873-1-AP (POLM antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 14873-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14873-1-AP (POLM antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human spleen using 14873-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14873-1-AP (POLM antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 14873-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14873-1-AP (POLM antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 14873-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 14873-1-AP (POLM antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 14873-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 14873-1-AP (POLM antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary using 14873-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14873-1-AP (POLM antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary using 14873-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14873-1-AP (POLM antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 14873-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14873-1-AP (POLM antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 14873-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14873-1-AP (POLM antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 14873-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14873-1-AP (POLM antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 14873-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14873-1-AP (POLM antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skin using 14873-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14873-1-AP (POLM antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin using 14873-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14873-1-AP (POLM antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 14873-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14873-1-AP (POLM antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 14873-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14873-1-AP (POLM antibody) at dilution of 1:50 (under 40x lens).

---

Tested Applications

• Positive WB detected in: Jurkat cells
• Positive IHC detected in: human spleen tissue, human brain tissue, human kidney tissue, human lung tissue, human ovary tissue, human skin tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14873-1-AP targets POLM in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: POLM fusion protein Ag6642
• Full Name: polymerase (DNA directed), mu
• Calculated Molecular Weight: 55 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC062590
• Gene Symbol: POLM
• Gene ID (NCBI): 27434
• RRID: AB_2237321
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.