PNO1 Polyclonal antibody

• Featured Product
• KD/KO Validated

PNO1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 21059-1-AP

Synonyms

KHRBP1, PNO1, RNA binding protein PNO1

---

Validation Data Gallery

• 

  WB analysis using 21059-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21059-1-AP (PNO1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 21059-1-AP

  WB result of PNO1 antibody (21059-1-AP; 1:800; incubated at room temperature for 1.5 hours) with sh-Control and sh-PNO1 transfected K-562 cells.

• 

  WB analysis of mouse spleen using 21059-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 21059-1-AP (PNO1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of L02 using 21059-1-AP

  IP result of anti-PNO1 (IP:21059-1-AP, 3ug; Detection:21059-1-AP 1:300) with L02 cells lysate 1500ug.

• 

  IHC staining of human liver cancer using 21059-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21059-1-AP (PNO1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human urothelial carcinoma using 21059-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 21059-1-AP (PNO1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human urothelial carcinoma using 21059-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 21059-1-AP (PNO1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: A2780 cells, mouse spleen tissue, K-562 cells, A549 cells
• Positive IP detected in: L02 cells
• Positive IHC detected in: human liver cancer tissue, human urothelial carcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below

Product Information

21059-1-AP targets PNO1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PNO1 fusion protein Ag14752
• Full Name: partner of NOB1 homolog (S. cerevisiae)
• Calculated Molecular Weight: 252 aa, 28 kDa
• Observed Molecular Weight: 28-35 kDa
• GenBank Accession Number: BC008304
• Gene Symbol: PNO1
• Gene ID (NCBI): 56902
• RRID: AB_10734582
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The function of PNO1 remains largely unknown in mammal. PNO1 plays a role in proteasome and ribosome neogenesis in yeast.

Publications

Species	Application	Title
human	WB	Sci Rep; PNO1 promotes the progression of osteosarcoma via TGF-β and YAP/TAZ pathway; Authors - Long Fang ; KD Validated