PLSCR4 Polyclonal antibody

PLSCR4 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 12630-1-AP

Synonyms

phospholipid scramblase 4, PL scramblase 4, PLSCR4, TRA1

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Validation Data Gallery

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  WB analysis of human heart using 12630-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 12630-1-AP (PLSCR4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 12630-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12630-1-AP (PLSCR4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 12630-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12630-1-AP (PLSCR4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 12630-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 12630-1-AP (PLSCR4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 12630-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 12630-1-AP (PLSCR4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: human heart tissue, human brain tissue, human placenta tissue, human liver tissue, mouse testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

12630-1-AP targets PLSCR4 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PLSCR4 fusion protein Ag3323
• Full Name: phospholipid scramblase 4
• Calculated Molecular Weight: 329 aa, 37 kDa
• Observed Molecular Weight: 37 kDa
• GenBank Accession Number: BC028354
• Gene Symbol: PLSCR4
• Gene ID (NCBI): 57088
• RRID: AB_2165948
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PLSCR4 (Phospholipid scramblase 4) belongs to the phospholipid scramblase family which is thought to mediate the loss of lipid asymmetry. Elimination of this lipid bilayer asymmetry is an important physiological process and appears to occur as a result of elevated intracellular calcium concentration, or occur with cell activation, injury, and apoptosis.PLSCR4 also plays a central role in the initiation of fibrin clot formation, in the activation of mast cells and in the recognition of apoptotic and injured cells by the reticuloendothelial system.

Publications

Species	Application	Title
mouse	WB	Reprod Sci; Phospholipid scramblase expression in the pregnant mouse uterus in LPS-induced preterm delivery.; Authors - McLean Kelley C KC