PLK5 Polyclonal antibody

PLK5 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 21299-1-AP

Synonyms

PLK5, PLK5P, polo like kinase 5 pseudogene, SgK384ps

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Validation Data Gallery

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  WB analysis using 21299-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21299-1-AP (PLK5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 21299-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 21299-1-AP (PLK5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 21299-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 21299-1-AP (PLK5 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 21299-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 21299-1-AP (PLK5 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 21299-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 21299-1-AP (PLK5 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 21299-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 21299-1-AP (PLK5 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 21299-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PLK5 antibody (21299-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: K-562 cells, mouse testis tissue, mouse kidney tissue
• Positive IHC detected in: human brain tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

The immunogen of 21299-1-AP is PLK5 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PLK5 fusion protein Ag15881
• Full Name: polo-like kinase 5 pseudogene
• Calculated Molecular Weight: 336 aa, 36 kDa
• Observed Molecular Weight: 55-60 kDa
• GenBank Accession Number: BC100792
• Gene Symbol: PLK5P
• Gene ID (NCBI): 126520
• RRID: AB_10732958
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PLK5, also named as PLK5P, is an inactive serine/threonine-protein kinase that plays a role in cell cycle progression and neuronal differentiation. Compared with mouse Plk5, the protein kinase domain of human PLK5 is truncated. This is the reason that the MW of mouse Plk5 is about 50-70 kDa, the MW of human PLK5 is about 32-40 kDa. PLK5 is located in the cytoplasm and nucleus(Uniprot). This antibody detects both PLK5 gene of human and mouse.

Publications

Species	Application	Title
human	WB,IHC,IF	Eur J Cell Biol; TWEAK/Fn14 signaling may function as a reactive compensatory mechanism against extracellular matrix accumulation in keloid fibroblasts; Authors - Yitian Zhang