PKIB Polyclonal antibody

PKIB Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 11942-1-AP

Synonyms

PKI beta, PKIB, PRKACN2

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Validation Data Gallery

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  IHC staining of human stomach cancer using 11942-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11942-1-AP (PKIB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 11942-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11942-1-AP (PKIB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 11942-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11942-1-AP (PKIB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 11942-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11942-1-AP (PKIB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 11942-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 11942-1-AP (PKIB antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse kidney using 11942-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11942-1-AP (PKIB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: human stomach cancer tissue, human gliomas tissue, mouse kidney tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11942-1-AP targets PKIB in IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PKIB fusion protein Ag2541
• Full Name: protein kinase (cAMP-dependent, catalytic) inhibitor beta
• Calculated Molecular Weight: 78 aa, 9 kDa
• GenBank Accession Number: BC036011
• Gene Symbol: PKIB
• Gene ID (NCBI): 5570
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The cAMP-dependent protein kinase inhibitor-β (PKIB) is also named as PRKACN2. PKIB is presumed to be one of the regulatory factors controlling the cAMP-dependent protein kinase A signaling pathway (PMID: 23224602). PKIB plays an important role in regulating the proliferation, migration, and even metastasis of osteosarcoma (PMID: 17195088). The expression of PKIB in the cytoplasm of tumor is closely related to pAkt and the triple negative breast cancer (PMID: 28387904).