PIWIL1 Polyclonal antibody

PIWIL1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 15659-1-AP

Synonyms

HIWI, MIWI, PIWI, piwi like 1 (Drosophila), Piwi like protein 1, PIWIL1

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Validation Data Gallery

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  WB analysis of human testis using 15659-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 15659-1-AP (PIWIL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 15659-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 15659-1-AP (PIWIL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 15659-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 15659-1-AP (PIWIL1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 15659-1-AP

  IP result of anti-PIWIL1 (IP:15659-1-AP, 4ug; Detection:15659-1-AP 1:500) with mouse testis tissue lysate 2400ug.

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  IHC staining of human testis using 15659-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 15659-1-AP (PIWIL1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 15659-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 15659-1-AP (PIWIL1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human prostate cancer using 15659-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15659-1-AP (PIWIL1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human prostate cancer using 15659-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15659-1-AP (PIWIL1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver cancer using 15659-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15659-1-AP (PIWIL1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 15659-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15659-1-AP (PIWIL1 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 15659-1-AP

  Immunofluorescent analysis of HepG2 cells using 15659-1-AP (PIWIL1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: human testis tissue, mouse testis tissue
• Positive IP detected in: mouse testis tissue
• Positive IHC detected in: human testis tissue, human liver cancer tissue, human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IF: See 3 publications below
• IP: See 2 publications below

Product Information

15659-1-AP targets PIWIL1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PIWIL1 fusion protein Ag8056
• Full Name: piwi-like 1 (Drosophila)
• Calculated Molecular Weight: 99 kDa
• Observed Molecular Weight: 85-95 kDa
• GenBank Accession Number: BC028581
• Gene Symbol: PIWIL1
• Gene ID (NCBI): 9271
• RRID: AB_2878163
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB,IF,IP	Development; MFN2 interacts with nuage-associated proteins and is essential for male germ cell development by controlling mRNA fate during spermatogenesis; Authors -
human	WB	Mol Ther Nucleic Acids; PIWIL1/piRNA-DQ593109 Regulates the Permeability of the Blood-Tumor Barrier via the MEG3/miR-330-5p/RUNX3 Axis.; Authors - Shuyuan Shen
mouse	WB,IF,IP	Development; MFN2 interacts with nuage-associated proteins and is essential for male germ cell development by controlling mRNA fate during spermatogenesis.; Authors - Xiaoli Wang
human	WB	J Transl Med; piRNA-14633 promotes cervical cancer cell malignancy in a METTL14-dependent m6A RNA methylation manner.; Authors - Qi Xie
human	IF	Prostate; High levels of PIWI-interacting RNAs are present in the small RNA landscape of prostate epithelium from vitamin D clinical trial specimens.; Authors - Bethany Baumann
mouse	WB	Andrologia; Secretions released from mesenchymal stem cells improve spermatogenesis restoration of cytotoxic treatment with busulfan in azoospermia mice.; Authors - Yi-Ting Cai