PIGZ Polyclonal antibody

PIGZ Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 17384-1-AP

Synonyms

GPI mannosyltransferase 4, GPI mannosyltransferase IV, GPI MT IV, hSMP3, PIG Z, PIGZ, SMP3, SMP3 homolog

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Validation Data Gallery

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  WB analysis of human brain using 17384-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 17384-1-AP (PIGZ antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 17384-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17384-1-AP (PIGZ antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 17384-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 17384-1-AP (PIGZ antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon using 17384-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 17384-1-AP (PIGZ antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human colon using 17384-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 17384-1-AP (PIGZ antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HepG2 using 17384-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 17384-1-AP (PIGZ antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: human brain tissue, HEK-293 cells, human testis tissue
• Positive IHC detected in: human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 1 publications below

Product Information

The immunogen of 17384-1-AP is PIGZ Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PIGZ fusion protein Ag11259
• Full Name: phosphatidylinositol glycan anchor biosynthesis, class Z
• Calculated Molecular Weight: 579 aa, 63 kDa
• Observed Molecular Weight: 63 kDa
• GenBank Accession Number: BC044640
• Gene Symbol: PIGZ
• Gene ID (NCBI): 80235
• RRID: AB_10697833
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB,IF	Phenomics; A Genome-Wide Association Study for Susceptibility to Axial Length in Highly Myopic Eyes; Authors - Qiang Lu
human	WB	J Biol Chem; Persistent ER stress causes GPI anchor deficit to convert a GPI-anchored prion protein (PrP) into pro-PrP via the ATF6-miR449c-5p-PIGV axis; Authors - JingFeng Li