PIF1 Polyclonal antibody

PIF1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 19006-1-AP

Synonyms

C15orf20, PIF, PIF1

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Validation Data Gallery

• 

  WB analysis of mouse thymus using 19006-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 19006-1-AP (PIF1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 19006-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 19006-1-AP (PIF1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse thymus tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

19006-1-AP targets PIF1 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PIF1 fusion protein Ag13522
• Full Name: PIF1 5'-to-3' DNA helicase homolog (S. cerevisiae)
• Calculated Molecular Weight: 70 kDa
• Observed Molecular Weight: 63-73 kDa
• GenBank Accession Number: BC018978
• Gene Symbol: PIF1
• Gene ID (NCBI): 80119
• RRID: AB_10598312
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PIF1, also named as ATP-dependent DNA helicase PIF1, is a 641 amino acid protein, which belongs to the helicase family. PIF1 as a DNA-dependent ATPase and 5'-3' DNA helicase is required for the maintenance of both mitochondrial and nuclear genome stability. PIF1 localizes in the nucleus and is involved in the maintenance of telomeric DNA. PIF1 exists as four isoforms. isoform 1 is a 70 kDa protein and isoform 2 is 63 kDa protein.

Publications

Species	Application	Title
human	WB	Cell Death Dis; CircNEIL3 mediates pyroptosis to influence lung adenocarcinoma radiotherapy by upregulating PIF1 through miR-1184 inhibition.; Authors - Ting Zhang