PIBF1 Polyclonal antibody

• Featured Product
• KD/KO Validated

PIBF1 Polyclonal Antibody for WB, IP, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Publications

6

Reviews (2)

Cat no : 14413-1-AP

Synonyms

C13orf24, CEP90, KIAA1008, PIBF, PIBF1, RP11 505F3.1

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Validation Data Gallery

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  WB analysis using 14413-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14413-1-AP (PIBF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14413-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14413-1-AP (PIBF1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 14413-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14413-1-AP (PIBF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 14413-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14413-1-AP (PIBF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 14413-1-AP

  IP result of anti-PIBF1 (IP:14413-1-AP, 4ug; Detection:14413-1-AP 1:1000) with HEK-293 cells lysate 1400 ug.

• 

  IF Staining of HEK-293 using 14413-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HEK-293 cells using PIBF1 antibody (14413-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Gamma Tubulin antibody (66320-1-Ig, Clone: 3F9H8, red).

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Tested Applications

• Positive WB detected in: HEK-293 cells, MCF-7 cells, K-562 cells, HeLa cells
• Positive IP detected in: HEK-293 cells
• Positive IF detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 2 publications below
• IF: See 5 publications below
• IP: See 1 publications below

Product Information

14413-1-AP targets PIBF1 in WB, IP, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PIBF1 fusion protein Ag5755
• Full Name: progesterone immunomodulatory binding factor 1
• Calculated Molecular Weight: 90 kDa
• Observed Molecular Weight: 90 kDa
• GenBank Accession Number: BC051911
• Gene Symbol: PIBF1
• Gene ID (NCBI): 10464
• RRID: AB_10858916
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PIBF1 is induced by the steroid hormone progesterone and plays a role in the maintenance of pregnancy. PIBF1 regulates multiple facets of the immune system to promote normal pregnancy including cytokine synthesis, natural killer (NK) cell activity, and arachidonic acid metabolism. Low serum levels of this protein have been associated with spontaneous pre-term labor in humans. PIBF1 may promote the proliferation, migration and invasion of glioma.

Publications

Species	Application	Title
human	WB, IP, IF	Elife; Centriolar satellites assemble centrosomal microcephaly proteins to recruit CDK2 and promote centriole duplication.; Authors - Andrew Kodani ; KD Validated
human	WB	J Cell Biol; A ciliopathy complex builds distal appendages to initiate ciliogenesis.; Authors - Dhivya Kumar ; KO Validated
human	IF	EMBO Rep; Zika virus alters centrosome organization to suppress the innate immune response.; Authors - Andrew Kodani
human	IF	PLoS Biol; The evolutionary conserved proteins CEP90, FOPNL, and OFD1 recruit centriolar distal appendage proteins to initiate their assembly; Authors - Pierrick Le Borgne ; KD Validated
human	IF	Cell Rep; CCDC57 Cooperates with Microtubules and Microcephaly Protein CEP63 and Regulates Centriole Duplication and Mitotic Progression.; Authors - H Kubra Gurkaslar
human	IF	EMBO J; Human SFI1 and Centrin form a complex critical for centriole architecture and ciliogenesis; Authors - Marine H Laporte

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Elisa (Verified Customer) (06-20-2022)

HEK293 cells stained for Hoechst (DNA marker, in green), Cep90 (mother centriole distal appendage marker, in magenta) and PCNT (pericentriolar matrix marker, in green). HEK293 cells were plated on Poly-lysine coated coverslips and fixed in cold methanol for 2' at -20C. Cells were then rehydrated with PBS for 5'. Membrane permeabilisation was then performed with 0.1% Triton + 0.1% Tween +0.01%SDS in PBS for 5'. Cells were finally incubated with blocking buffer (5% BSA+ 0.1% Tween in PBS) for 30' at RT. Primary antibody was diluted in blocking buffer 1:200 and incubated for 1h at room temperature. Alexa-555-Anti-rabbit was used as secondary antibody (1:600 dilution) (1h at room temperature). Cep90 antibody recognises clearly dots at the centrosome (identified by the presence of PCNT).

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:300
• Cell Tissue Type: HEK293 cells (Human embryonic kidney 293 cells)

PIBF1 Antibody Immunofluorescence validation (1:300 dilution) in HEK293 cells (Human embryonic kidney 293 cells) (Cat no:14413-1-AP)

FH

Pierrick (Verified Customer) (10-24-2019)

Antibody mostly used in wester blot and work well at 1/1000 dilution on RPE1 and HEK293 sample

• Applications: Western Blot, Immunofluorescence, Immunoprecipitation,
• Primary Antibody Dilution: PIBF1 rabbit: if 1/500 WB 1/1000
• Cell Tissue Type: RPE1, HEK290, HeLa Kyoto