Elafin/Skalp Polyclonal antibody

Elafin/Skalp Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IF, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 15963-1-AP

Synonyms

cementoin, Elafin, Elastase specific inhibitor, ESI, Peptidase inhibitor 3, PI 3, PI3, Protease inhibitor WAP3, SKALP, WAP3, WFDC14

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Validation Data Gallery

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  IHC staining of human skin cancer using 15963-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 15963-1-AP (Elafin/Skalp antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin cancer using 15963-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 15963-1-AP (Elafin/Skalp antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human oesophagus cancer using 15963-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 15963-1-AP (Elafin/Skalp antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human oesophagus cancer using 15963-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 15963-1-AP (Elafin/Skalp antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human oesophagus using 15963-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 15963-1-AP (Elafin/Skalp antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human oesophagus using 15963-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 15963-1-AP (Elafin/Skalp antibody at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 15963-1-AP

  Immunofluorescent analysis of HeLa cells using 15963-1-AP (Elafin/Skalp antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive IHC detected in: human skin cancer tissue, human oesophagus tissue, human oesophagus cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 4 publications below

Product Information

15963-1-AP targets Elafin/Skalp in IF, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Elafin/Skalp fusion protein Ag8735
• Full Name: peptidase inhibitor 3, skin-derived
• Calculated Molecular Weight: 117 aa, 12 kDa
• GenBank Accession Number: BC010952
• Gene Symbol: PI3
• Gene ID (NCBI): 5266
• RRID: AB_2878201
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IHC	Ther Adv Med Oncol; Development and external validation of a composite immune-clinical prognostic model associated with EGFR mutation in East-Asian patients with lung adenocarcinoma.; Authors - Chengming Liu
pig	IHC	Anim Biosci; Spatiotemporal expression and regulation of peptidase inhibitor 3 and secretory leukocyte protease inhibitor at the maternal-fetal interface in pigs; Authors - Soohyung Lee
human	IHC	Int J Gen Med; FAM3B Serves as a Biomarker for the Development and Malignancy of Oral Lichen Planus.; Authors - Wenfang Wang
human	IHC	Front Endocrinol (Lausanne); Elafin is related to immune infiltration and could predict the poor prognosis in ovarian cancer; Authors - Weiyu Lu