PHKB Polyclonal antibody

• Featured Product
• KD/KO Validated

PHKB Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 13400-1-AP

Synonyms

PHKB, phosphorylase kinase, beta

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Validation Data Gallery

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  WB analysis of HepG2 using 13400-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13400-1-AP (PHKB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 13400-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13400-1-AP (PHKB antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 13400-1-AP

  IP result of anti-PHKB (IP:13400-1-AP, 4ug; Detection:13400-1-AP 1:500) with mouse heart tissue lysate 1680 ug.

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  IHC staining of human skeletal muscle using 13400-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 13400-1-AP (PHKB Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse liver using 13400-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 13400-1-AP (PHKB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 13400-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 13400-1-AP (PHKB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skeletal muscle using 13400-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 13400-1-AP (PHKB Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse skeletal muscle using 13400-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 13400-1-AP (PHKB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 13400-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13400-1-AP (PHKB Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 13400-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13400-1-AP (PHKB Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: HepG2 cells, Jurkat cells, K-562 cells, LNCaP cells, mouse heart tissue, rat heart tissue, rat skeletal muscle tissue, mouse skeletal muscle tissue
• Positive IP detected in: mouse heart tissue
• Positive IHC detected in: mouse liver tissue, human skeletal muscle tissue, human liver tissue, mouse skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IP: See 1 publications below

Product Information

13400-1-AP targets PHKB in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PHKB fusion protein Ag4240
• Full Name: phosphorylase kinase, beta
• Calculated Molecular Weight: 1086 aa, 124 kDa
• Observed Molecular Weight: 124 kDa
• GenBank Accession Number: BC033657
• Gene Symbol: PHKB
• Gene ID (NCBI): 5257
• RRID: AB_2237183
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PHKB gene encodes phosphorylase kinase subunit beta involved in glycan biosynthesis and glycogen metabolism. PHKB activity is regulated by phosphorylation of various serine residues, and catalyzes the phosphorylation of serine in certain substrates, including troponin I. Phosphorylase kinase (PhK) complex, composed of alpha, beta, gamma, and delta subunits, stimulates energy production from glycogen in the cascade activation of glycogenolysis. Its large homologous alpha and beta subunits regulate the activity of the catalytic gamma subunit. Defects in PHKB are the cause of glycogen storage disease type 9B (GSD9B) also known as phosphorylase kinase deficiency of liver and muscle (PKD), characterized by hepathomegaly, only slightly elevated transaminases and plasma lipids, clinical improvement with increasing age, and remarkably no clinical muscle involvement.

Publications

Species	Application	Title
mouse	IP, WB	Mol Endocrinol; 20-HETE induces hyperglycemia through the cAMP/PKA-PhK-GP pathway.; Authors - Lai Guangrui G
mouse	WB	iScience; Loss of RNA-Binding Protein Sfpq Causes Long-Gene Transcriptopathy in Skeletal Muscle and Severe Muscle Mass Reduction with Metabolic Myopathy.; Authors - Motoyasu Hosokawa
human	WB,IHC	J Cancer Res Clin Oncol; Phosphorylase kinase β affects colorectal cancer cell growth and represents a novel prognostic biomarker.; Authors - Guanghui Wang ; KD Validated