PHF2 Polyclonal antibody

PHF2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 24624-1-AP

Synonyms

GRC5, JHDM1E, KIAA0662, PHD finger protein 2, PHF2

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Validation Data Gallery

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  WB analysis of Jurkat using 24624-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 24624-1-AP (PHF2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 24624-1-AP

  IP result of anti-PHF2 (IP:24624-1-AP, 4ug; Detection:24624-1-AP 1:500) with HeLa cells lysate 1800ug.

• 

  IHC staining of human cervical cancer using 24624-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 24624-1-AP (PHF2 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human cervical cancer using 24624-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 24624-1-AP (PHF2 Antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: Jurkat cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

The immunogen of 24624-1-AP is PHF2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PHF2 fusion protein Ag20257
• Full Name: PHD finger protein 2
• Calculated Molecular Weight: 1101 aa, 121 kDa
• Observed Molecular Weight: 150 kDa
• GenBank Accession Number: BC152437
• Gene Symbol: PHF2
• Gene ID (NCBI): 5253
• RRID: AB_2879644
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PHD finger protein 2 (PHF2), also known as JHDM1E (Jumonji C domain-containing histone demethylase 1E) or GRC5, is a 1101 amino acid protein, which contains one JmjC domain and one PHD-type zinc finger. PHF2 belongs to the JHDM1 histone demethylase family and localizes in the nucleus. PHF2 demethylases both histones and non-histione proteins. PHF2 is widely expressed in various tissues and exists as two alternative splicing isoforms. The gene encoding PHF2 lies in the candidate region for hereditary sensory neuropathy type I (HSN1), a disorder characterized by sensory dysfunction

Publications

Species	Application	Title
human	WB	Am J Hum Genet; High-throughput identification of regulatory elements and functional assays to uncover susceptibility genes for nasopharyngeal carcinoma; Authors - Tong-Min Wang