CoraLite®594-conjugated PGD Monoclonal antibody

PGD Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2A10G4

Cat no : CL594-67916

Synonyms

6PGD, PGD, PGDH, phosphogluconate dehydrogenase

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Validation Data Gallery

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  IF Staining of HepG2 using CL594-67916

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite®594 PGD antibody (CL594-67916, Clone: 2A10G4 ) at dilution of 1:200.

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Tested Applications

• Positive IF detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67916 targets PGD in IF applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PGD fusion protein Ag6871
• Full Name: phosphogluconate dehydrogenase
• Calculated Molecular Weight: 53 kDa
• Observed Molecular Weight: 53 kDa, 45 kDa
• GenBank Accession Number: BC000368
• Gene Symbol: PGD
• Gene ID (NCBI): 5226
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PGD, also named as PGDH, belongs to the 6-phosphogluconate dehydrogenase family. PGD catalyses the oxidative decarboxylation of 6-phosphogluconate to ribulose 5-phosphate in the context of the oxidative part of the pentose phosphate pathway. PGD is important for the production of NADPH, which is necessary for reductive biosynthesis, such as the formation of lipids and nucleotides, and the activity of enzymes involved in maintaining cell integrity, in combatting oxidative stress and in the first line of immunological defence. (PMID: 35234135)