CoraLite® Plus 488-conjugated PFKFB3-Specific Polyclonal antibody

• Featured Product
• KD/KO Validated

PFKFB3-Specific Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-13763

Synonyms

6PF 2 K/Fru 2,6 P2ase 3, FLJ37326, IPFK2, PFK/FBPase 3, PFK2, PFKFB3

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Validation Data Gallery

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  IF Staining of HepG2 using CL488-13763

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 PFKFB3-Specific antibody (CL488-13763) at dilution of 1:200.

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Tested Applications

• Positive IF detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-13763 targets PFKFB3-Specific in IF applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PFKFB3-Specific fusion protein Ag4744
• Full Name: 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3
• Calculated Molecular Weight: 520 aa, 60 kDa
• Observed Molecular Weight: 58 kDa
• GenBank Accession Number: BC040482
• Gene Symbol: PFKFB3
• Gene ID (NCBI): 5209
• RRID: AB_2919078
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PFKFB3, also named as NY-REN-56 and iPFK-2, plays a role in glucose metabolism. It synthesis and degradation of fructose 2,6-bisphosphate. Endogenously generated adenosine cooperates with bacterial components to increase PFKFB3 isozyme activity, resulting in greater fructose 2,6-bisphosphate accumulation. PFKFB3 is required for increased growth, metabolic activity and is regulated through active JAK2 and STAT5. This antibody is specific to PFKFB3.