PELP1 Polyclonal antibody

PELP1 Polyclonal Antibody for IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF, ELISA

Conjugate

Unconjugated

Cat no : 27239-1-AP

Synonyms

HMX3, MNAR, P160, PELP1, Transcription factor HMX3

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Validation Data Gallery

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  IF Staining of HeLa using 27239-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 27239-1-AP (PELP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive IF detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 27239-1-AP is PELP1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PELP1 fusion protein Ag25729
• Full Name: proline, glutamate and leucine rich protein 1
• Calculated Molecular Weight: 1130 aa, 120 kDa
• GenBank Accession Number: BC069058
• Gene Symbol: PELP1
• Gene ID (NCBI): 27043
• RRID: AB_2918120
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PELP1 was first identified as a 160 kDa protein in a screen for Src homology 2 (SH2) domain-binding proteins. PELP1 is overexpressed in 60-80% of breast tumors and plays important roles in both ER genomic and non-genomic signaling. In vivo, PELP1 subcellular localization is primarily nuclear in normal breast tissue, but it is localized to the cytoplasm in about 40% of invasive breast tumors. In the nucleus, PELP1 interacts with a number of transcription factors. The proto-oncogenic functions of PELP1 involve different cellular processes including epigenetic modifications leading to ER transactivation and breast cancer progression. Furthermore, PELP1 activates kinase cascades in the cytoplasm such as MAPK activation via c-Src and PI3K signaling.