PCP4L1 Polyclonal antibody

• Featured Product
• KD/KO Validated

PCP4L1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 25933-1-AP

Synonyms

IQM1, PCP4 like protein 1, PCP4L1, Purkinje cell protein 4 like 1

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Validation Data Gallery

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  WB analysis of mouse brain using 25933-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25933-1-AP (PCP4L1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 25933-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25933-1-AP (PCP4L1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 25933-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25933-1-AP (PCP4L1 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 25933-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25933-1-AP (PCP4L1 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

25933-1-AP targets PCP4L1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PCP4L1 fusion protein Ag23061
• Full Name: Purkinje cell protein 4 like 1
• Calculated Molecular Weight: 68 aa, 7 kDa
• Observed Molecular Weight: 8-15 kDa
• GenBank Accession Number: BC028905
• Gene Symbol: PCP4L1
• Gene ID (NCBI): 654790
• RRID: AB_2880301
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PCP4L1 (Purkinje cell protein 4-like 1) is a small neuronal IQ motif protein closely related to the calmodulin-binding protein Pcp4/PEP-19 (PMID: 22458599).

Publications

Species	Application	Title
mouse	WB	Genome Biol; A novel antiviral lncRNA, EDAL, shields a T309 O-GlcNAcylation site to promote EZH2 lysosomal degradation.; Authors - Baokun Sui ; KD Validated
mouse	IHC	J Gen Virol; lncRNA EDAL restricts rabies lyssavirus replication in a cell-specific and infection route-dependent manner.; Authors - Baokun Sui
mouse	WB	J Proteome Res; Multiplexed Quantitative Proteomics Reveals Proteomic Alterations in Two Rodent Traumatic Brain Injury Models; Authors - Junho Park