PCIF1 Polyclonal antibody

PCIF1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

5

Reviews (1)

Cat no : 16082-1-AP

Synonyms

C20orf67, PCIF1

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Validation Data Gallery

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  WB analysis using 16082-1-AP

  Ramos cells were subjected to SDS PAGE followed by western blot with 16082-1-AP (PCIF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16082-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16082-1-AP (PCIF1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 16082-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16082-1-AP (PCIF1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293T using 16082-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 16082-1-AP (PCIF1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 16082-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16082-1-AP (PCIF1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 16082-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16082-1-AP (PCIF1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Ramos cells, HepG2 cells, Jurkat cells, HEK-293T cells
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

16082-1-AP targets PCIF1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PCIF1 fusion protein Ag9080
• Full Name: PDX1 C-terminal inhibiting factor 1
• Calculated Molecular Weight: 704 aa, 81 kDa
• Observed Molecular Weight: 81 kDa
• GenBank Accession Number: BC013365
• Gene Symbol: PCIF1
• Gene ID (NCBI): 63935
• RRID: AB_2159996
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human,mouse	WB,IP	Mol Cell; RNA Pol II preferentially regulates ribosomal protein expression by trapping disassociated subunits; Authors - Yuanjun Li
human	WB	Nat Commun; HIV reprograms host m 6 Am RNA methylome by viral Vpr protein-mediated degradation of PCIF1; Authors - Qiong Zhang
pig	WB	Cell Rep; Alphaherpesvirus US3 protein-mediated inhibition of the m6A mRNA methyltransferase complex.; Authors - Robert J J Jansens
human,mouse	WB,IF	EMBO J; Role of PCIF1-mediated 5\'-cap N6-methyladeonsine mRNA methylation in colorectal cancer and anti-PD-1 immunotherapy; Authors - Lingling Wang
human	WB	Proc Natl Acad Sci U S A; PCIF1-mediated deposition of 5'-cap N6,2'-O-dimethyladenosine in ACE2 and TMPRSS2 mRNA regulates susceptibility to SARS-CoV-2 infection; Authors - Lingling Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Anirban (Verified Customer) (01-25-2023)

blocking 1 hr in 5% milk/TBST. Primary ab- 1/1000 in 3% BSA/TBST at 4 deg for 16 hrs, Secondary ab-1/5000 in 5% milk/TBST.

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: 786-O, 769p

PCIF1 Antibody Western Blot validation (1/1000 dilution) in 786-O, 769p (Cat no:16082-1-AP)