PCDHA5 Polyclonal antibody

PCDHA5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15270-1-AP

Synonyms

CNR6, CNRN6, CNRS6, CRNR6, PCDH alpha 5, PCDH ALPHA5, PCDHA5, protocadherin alpha 5

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Validation Data Gallery

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  WB analysis of mouse brain using 15270-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15270-1-AP (PCDHA5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 15270-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15270-1-AP (PCDHA5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 15270-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15270-1-AP (PCDHA5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse cerebellum using 15270-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 15270-1-AP (PCDHA5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 15270-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 15270-1-AP (PCDHA5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 15270-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 15270-1-AP (PCDHA5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 15270-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 15270-1-AP (PCDHA5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat cerebellum using 15270-1-AP

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 15270-1-AP (PCDHA5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat cerebellum using 15270-1-AP

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 15270-1-AP (PCDHA5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 15270-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15270-1-AP (PCDHA5 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 15270-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15270-1-AP (PCDHA5 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung using 15270-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 15270-1-AP (PCDHA5 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 15270-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 15270-1-AP (PCDHA5 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse brain tissue, human brain tissue
• Positive IHC detected in: mouse cerebellum tissue, human gliomas tissue, rat cerebellum tissue, human brain tissue, human lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15270-1-AP targets PCDHA5 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PCDHA5 fusion protein Ag7376
• Full Name: protocadherin alpha 5
• Calculated Molecular Weight: 102 kDa
• Observed Molecular Weight: 102 kDa
• GenBank Accession Number: BC033735
• Gene Symbol: PCDHA5
• Gene ID (NCBI): 56143
• RRID: AB_2283415
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.