PBRM1 Polyclonal antibody

PBRM1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA, Dot blot

Conjugate

Unconjugated

Publications

3

Cat no : 12563-1-AP

Synonyms

BAF180, BRG1 associated factor 180, hPB1, PB1, PBRM1, polybromo 1, Polybromo 1D, Protein polybromo 1

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Validation Data Gallery

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  WB analysis of Jurkat using 12563-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12563-1-AP (PBRM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IP: See 1 publications below

Product Information

12563-1-AP targets PBRM1 in WB, IP, ELISA, Dot blot applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PBRM1 fusion protein Ag3148
• Full Name: polybromo 1
• Calculated Molecular Weight: 180 kDa
• Observed Molecular Weight: 180-195 kDa, 100 kDa
• GenBank Accession Number: BC015323
• Gene Symbol: PBRM1
• Gene ID (NCBI): 55193
• RRID: AB_2877865
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PBRM1, also named hPB1 BAF180 and Polybromo-1D, acts as a negative regulator of cell proliferation. PBRM1 is the second major mutated clear cell RCC cancer gene after VHL. PBRM1 loss is associated with low grade features.

Publications

Species	Application	Title
	Dot blot	Protein Expr Purif; Heterologous expression of the human polybromo-1 protein in the methylotrophic yeast Pichia pastoris.; Authors - Sarah E Hopson
human	WB,IP	Cell Death Dis; The RBPJ/DAPK3/UBE3A signaling axis induces PBRM1 degradation to modulate the sensitivity of renal cell carcinoma to CDK4/6 inhibitors.; Authors - Wentao Liu
human	WB	J Am Heart Assoc; Aging-Associated ALKBH5-m6A Modification Exacerbates Doxorubicin-Induced Cardiomyocyte Apoptosis Via AT-Rich Interaction Domain 2; Authors - Runtai Chen