PAPOLG Polyclonal antibody

PAPOLG Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24284-1-AP

Synonyms

Neo PAP, Neo poly(A) polymerase, PAP gamma, PAP2, PAPG, PAPOLG, poly(A) polymerase gamma, SRP RNA 3 adenylating enzyme

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Validation Data Gallery

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  WB analysis of HeLa using 24284-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24284-1-AP (PAPOLG Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 24284-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 24284-1-AP (PAPOLG Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 24284-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 24284-1-AP (PAPOLG Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 24284-1-AP

  IP result of anti-PAPOLG (IP:24284-1-AP, 4ug; Detection:24284-1-AP 1:1000) with HeLa cells lysate 3200ug.

• 

  IHC staining of human testis using 24284-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24284-1-AP (PAPOLG Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 24284-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24284-1-AP (PAPOLG Antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HeLa using 24284-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 24284-1-AP (PAPOLG antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, MCF-7 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24284-1-AP is PAPOLG Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PAPOLG fusion protein Ag16208
• Full Name: poly(A) polymerase gamma
• Calculated Molecular Weight: 736 aa, 83 kDa
• Observed Molecular Weight: 83 kDa
• GenBank Accession Number: BC111701
• Gene Symbol: PAPOLG
• Gene ID (NCBI): 64895
• RRID: AB_2879471
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PAPOLG, also named as Neo-poly(A) polymerase or Polynucleotide adenylyltransferase gamma, is a 736 amino acid protein, which belongs to the poly(A) polymerase family. PAPOLG is expressed predominantly in testis, and weakly in other tissues. PAPOLG is responsible for the post-transcriptional adenylation of the 3'-terminal of mRNA precursors and several small RNAs including signal recognition particle (SRP) RNA, nuclear 7SK RNA, U2 small nuclear RNA, and ribosomal 5S RNA.