PANX2 Polyclonal antibody

• Featured Product
• KD/KO Validated

PANX2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 26604-1-AP

Synonyms

hPANX2, pannexin 2, PANX2, PX2

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Validation Data Gallery

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  WB analysis of mouse lung using 26604-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 26604-1-AP (PANX2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 26604-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 26604-1-AP (PANX2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 26604-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 26604-1-AP (PANX2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skeletal muscle using 26604-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 26604-1-AP (PANX2 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 26604-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 26604-1-AP (PANX2 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human kidney using 26604-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26604-1-AP (PANX2 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 26604-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26604-1-AP (PANX2 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse lung tissue, mouse spleen tissue, PC-3 cells
• Positive IHC detected in: human skeletal muscle tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below

Product Information

The immunogen of 26604-1-AP is PANX2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PANX2 fusion protein Ag24256
• Full Name: pannexin 2
• Calculated Molecular Weight: 677 aa, 74 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC101023
• Gene Symbol: PANX2
• Gene ID (NCBI): 56666
• RRID: AB_2880572
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Pannexins (Panx) are proteins homologous to the invertebrate gap junction proteins called innexins (Inx) and are traditionally described as transmembrane channels. Three distinct Panx paralogs (Panx1, Panx2 and Panx3) have been identified in vertebrates. Previous reports on Panx expression and functionality are focused primarily on Panx1 and Panx3 proteins. The exact function of Panx2 remains elusive. Catalog#26604-1-AP specially recognizes the 70 kDa Panx2.

Publications

Species	Application	Title
human	WB	Nan Fang Yi Ke Da Xue Xue Bao; [Down-regulation of pannexin 2 channel enhances cisplatin-induced apoptosis in testicular cancer I-10 cells].; Authors - Yanxue Yao ; KD Validated