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CoraLite® Plus 488-conjugated PAIP1 Monoclonal antibody

PAIP1 Monoclonal Antibody for IF, FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rabbit

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2H9D4

Cat no : CL488-68042

Synonyms

PABP interacting protein 1, PAIP 1, PAIP1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated PAIP1 Monoclonal ant (CL488-68042)

    IF Staining of HeLa using CL488-68042

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 PAIP1 antibody (CL488-68042, Clone: 2H9D4 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated PAIP1 Monoclonal ant (CL488-68042)

    FC experiment of HeLa using CL488-68042

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human PAIP1 (CL488-68042, Clone:2H9D4) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68042 targets PAIP1 in IF, FC (Intra) applications and shows reactivity with Human, mouse, rabbit samples.

Tested Reactivity Human, mouse, rabbit
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen PAIP1 fusion protein Ag16773
Full Name poly(A) binding protein interacting protein 1
Calculated Molecular Weight 70 kDa
Observed Molecular Weight40-45kDa, 62kDa
GenBank Accession NumberBC005295
Gene Symbol PAIP1
Gene ID (NCBI) 10605
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PAIP1 is a PABP-binding protein that contains two distinct PABP-binding motifs (PAMs). PAM1 binds to RNA recognition motif 2 in the N terminus of PABP; PAM2, which is a conserved region comprising approximately 15 amino acids, binds to the PABC domain of PABP. PAIP1 acts as a coactivator in the regulation of translation initiation of poly(A)-containing mRNAs.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 PAIP1 antibody CL488-68042Download protocol
FC protocol for CL Plus 488 PAIP1 antibody CL488-68042Download protocol
Standard Protocols
Click here to view our Standard Protocols