PAFAH Polyclonal antibody

PAFAH Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29304-1-AP

Synonyms

Group VIIA phospholipase A2, gVIIA PLA2, LDL PLA(2), LDL PLA2, PAF 2 acylhydrolase, PAF acetylhydrolase, PAFAH, PLA2G7

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Validation Data Gallery

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  WB analysis using 29304-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29304-1-AP (PAFAH antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 29304-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 29304-1-AP (PAFAH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: RAW 264.7 cells, mouse liver tissue, rat liver tissue
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29304-1-AP targets PAFAH in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PAFAH fusion protein Ag30947
• Full Name: phospholipase A2, group VII (platelet-activating factor acetylhydrolase, plasma)
• Calculated Molecular Weight: 50 kDa
• Observed Molecular Weight: 29-30 kDa, 45-55 kDa
• GenBank Accession Number: BC038452
• Gene Symbol: PLA2G7
• Gene ID (NCBI): 7941
• RRID: AB_2918276
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Platelet-activating factor acetylhydrolase (PAFAH) Ib consists of homo- or heterodimers of two PLA2 subunits α1 and α2, and a dimer of a third subunit, LIS1. PAFAH Ib was first discovered by its ability to hydrolyze the extracellular signaling lipid, PAF, which plays a role in platelet activation and inflammation. LIS1, the causative agent of the fatal brain disorder Miller-Dieker lissencephaly, functions independently of the α subunits to regulate the activity of cytoplasmic dynein. LIS1 is a highly conserved protein involved in dynein-mediated processes including nuclear and neuronal migration, centrosomal function, and mitotic spindle orientation. (PMID: 20624900, PMID: 23262398)