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  • KD/KO Validated

PAF1 Polyclonal antibody

PAF1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15441-1-AP

Synonyms

F23149_1, hPAF1, PAF1, PD2



Tested Applications

Positive WB detected inHepG2 cells, HeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman ovary tumor tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15441-1-AP targets PAF1 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PAF1 fusion protein Ag7212
Full Name Paf1, RNA polymerase II associated factor, homolog (S. cerevisiae)
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 80 kDa
GenBank Accession NumberBC000017
Gene Symbol PAF1
Gene ID (NCBI) 54623
RRIDAB_2174457
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PAF1, also known as hPAF1 or PD2, is a PAF (RNA Polymerase II Associated Factor) complex subunit. Paf1 functions primarily to modulates transcript elongation. PAF1 occupies transcriptional enhancers and restrains hyperactivation of a subset of these enhancers(PMID: 28860207). The PAF complex also has a role in histone monoubiquitination. Paf1 is overexpressed in pancreatic cancer cells and has demonstrated potential oncogenic property. The MW of this protein is 80 kDa, and this antibody specially recognises the 80 kDa protein.

Protocols

Product Specific Protocols
WB protocol for PAF1 antibody 15441-1-APDownload protocol
IHC protocol for PAF1 antibody 15441-1-APDownload protocol
IP protocol for PAF1 antibody 15441-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Autophagy

The Paf1 complex transcriptionally regulates the mitochondrial-anchored protein Atg32 leading to activation of mitophagy.

Authors - Liangde Zheng
  • KD Validated
humanWB

J Cell Biol

Phase separation and toxicity of C9orf72 poly(PR) depends on alternate distribution of arginine.

Authors - Chen Chen
humanWB

Radiat Oncol

Novel role of PAF1 in attenuating radiosensitivity in cervical cancer by inhibiting IER5 transcription.

Authors - Jing-Jie Zheng
humanWB

ACS Pharmacol Transl Sci

Identification of PDCD2 as a Candidate Target of Andrographolide That Arrests the Tumor Cell Cycle by Human Proteome-Scale Screening

Authors - Lei Wang
humanWB

Sci Adv

CDK12 and Integrator-PP2A complex modulates LEO1 phosphorylation for processive transcription elongation

Authors - Min Qiu
humanWB

Cell

SARS-CoV-2 variants evolve convergent strategies to remodel the host response

Authors - Mehdi Bouhaddou