PACSIN3 Polyclonal antibody

PACSIN3 Polyclonal Antibody for WB, IF, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, FC, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10639-1-AP

Synonyms

Endophilin I, PACSIN3, SDPIII

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Validation Data Gallery

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  WB analysis of HeLa using 10639-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10639-1-AP (PACSIN3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 10639-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 10639-1-AP (PACSIN3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 10639-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10639-1-AP (PACSIN3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 10639-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10639-1-AP (PACSIN3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 10639-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 10639-1-AP (PACSIN3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 10639-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10639-1-AP (PACSIN3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 10639-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10639-1-AP (PACSIN3 antibody) at dilution of 1:100 (under 25x lens).

• 

  IHC staining of human heart using 10639-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10639-1-AP (PACSIN3 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 10639-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10639-1-AP (PACSIN3 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 10639-1-AP

  Immunofluorescent analysis of HeLa cells using 10639-1-AP (PACSIN3 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of MCF-7 using 10639-1-AP

  1X10^6 MCF-7 cells were stained with 0.2ug PACSIN3 antibody (10639-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Tested Applications

• Positive WB detected in: HeLa cells, mouse heart tissue, mouse liver tissue, mouse lung tissue, mouse skeletal muscle tissue, rat brain tissue
• Positive IHC detected in: human breast cancer tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells
• Positive FC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

10639-1-AP targets PACSIN3 in WB, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PACSIN3 fusion protein Ag1006
• Full Name: protein kinase C and casein kinase substrate in neurons 3
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: BC007914
• Gene Symbol: PACSIN3
• Gene ID (NCBI): 29763
• RRID: AB_2159453
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.