CoraLite® Plus 488-conjugated PA2G4 Monoclonal antibody

• Featured Product
• KD/KO Validated

PA2G4 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2a

Reactivity

human, rat, mouse

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3G10D9

Cat no : CL488-66055

Synonyms

EBP1, ErbB3 binding protein 1, HG4 1, p38 2G4, PA2G4

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Validation Data Gallery

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  IF Staining of HeLa using CL488-66055

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CL488-66055 (PA2G4 antibody) at dilution of 1:100.

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Tested Applications

• Positive IF detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL488-66055 is PA2G4 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, rat, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: PA2G4 fusion protein Ag7850
• Full Name: proliferation-associated 2G4, 38kDa
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 38-42 kDa, 48 kDa
• GenBank Accession Number: BC001951
• Gene Symbol: PA2G4
• Gene ID (NCBI): 5036
• RRID: AB_2883242
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PA2G4 (EBP1), first identified as an ErbB3 binding protein, is a 38 kDa protein that is widely expressed in cultured cells and tissues. The presence of several functional domains such as a nuclear localization sequence (NLS), LxxLL and LxCxE suggests the involvement of Ebp1 in cell signaling pathways and gene transcription regulation. This antibody is a mouse mono-clonal antibody raised against full length PA2G4 of human origin.