PA2G4 Polyclonal antibody

• Featured Product
• KD/KO Validated

PA2G4 Polyclonal Antibody for WB, IP, IF, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, FC, RIP, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 15348-1-AP

Synonyms

EBP1, ErbB3 binding protein 1, HG4 1, p38 2G4, PA2G4

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Validation Data Gallery

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  WB analysis of HEK-293 using 15348-1-AP

  WB result of PA2G4 antibody (15348-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PA2G4 transfected HEK-293 cells.

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  WB analysis using 15348-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15348-1-AP (PA2G4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat spleen using 15348-1-AP

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 15348-1-AP (PA2G4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 15348-1-AP

  IP result of anti-PA2G4 (IP:15348-1-AP, 4ug; Detection:15348-1-AP 1:1000) with Jurkat cells lysate 3000ug.

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  IHC staining of human breast cancer using 15348-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15348-1-AP (PA2G4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 15348-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15348-1-AP (PA2G4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 15348-1-AP

  Immunofluorescent analysis of MCF-7 cells, using PA2G4 antibody 15348-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  FC experiment of MCF-7 using 15348-1-AP

  1X10^6 MCF-7 cells were stained with 0.2ug PA2G4 antibody (15348-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Tested Applications

• Positive WB detected in: HEK-293T cells, HEK-293 cells, rat spleen tissue, HL-60 cells, HeLa cells, Jurkat cells, MCF-7 cells, PC-3 cells
• Positive IP detected in: Jurkat cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: MCF-7 cells
• Positive FC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 2 publications below
• IP: See 1 publications below
• RIP: See 1 publications below

Product Information

15348-1-AP targets PA2G4 in WB, IP, IF, FC, RIP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PA2G4 fusion protein Ag7587
• Full Name: proliferation-associated 2G4, 38kDa
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 42 kDa, 48 kDa
• GenBank Accession Number: BC001951
• Gene Symbol: PA2G4
• Gene ID (NCBI): 5036
• RRID: AB_2156602
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PA2G4 (EBP1), first identified as an ErbB3 binding protein, is a 38 kDa protein that is widely expressed in cultured cells and tissues. The presence of several functional domains such as a nuclear localization sequence (NLS), LxxLL and LxCxE suggests the involvement of Ebp1 in cell signaling pathways and gene transcription regulation. This antibody is a rabbit polyclonal antibody raised against full length PA2G4 of human origin.

Publications

Species	Application	Title
human	WB,IHC,IF,RIP	Cell Biosci; PA2G4 promotes the metastasis of hepatocellular carcinoma by stabilizing FYN mRNA in a YTHDF2-dependent manner.; Authors - Sheng Sun ; KD Validated
human	WB,IHC	FASEB J; Evidence for a fragile X messenger ribonucleoprotein 1 (FMR1) mRNA gain-of-function toxicity mechanism contributing to the pathogenesis of fragile X-associated premature ovarian insufficiency; Authors - Roseanne Rosario
		Methods Enzymol; Selective ribosome profiling as a tool to study interactions of translating ribosomes in mammalian cells; Authors - Manuel Günnigmann
human	WB,IF,IP	J Dent Sci; Proliferation-associated 2G4 P48 is stabilized by malignant T-cell amplified sequence 1 and promotes the proliferation of head and neck squamous cell carcinoma; Authors - Legang Sun