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OXA1L Monoclonal antibody

OXA1L Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

4A2D9

Cat no : 66128-1-Ig

Synonyms

Hsa, OXA1 like protein, OXA1Hs, OXA1L



Tested Applications

Positive WB detected inT-47D cells, NIH/3T3 cells, HEK-293 cells, HeLa cells, HepG2 cells, L02 cells, Jurkat cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 4 publications below

Product Information

66128-1-Ig targets OXA1L in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen OXA1L fusion protein Ag13819
Full Name oxidase (cytochrome c) assembly 1-like
Calculated Molecular Weight 437 aa, 49 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberBC001669
Gene Symbol OXA1L
Gene ID (NCBI) 5018
RRIDAB_2881527
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

OXA1L/Oxa1 is a member of the conserved Oxa1/YidC/Alb3 protein family and is required for cytochrome c oxidase assembly. The OXA1L protein was initially identified in yeast proteins involved in the biogenesis of the mitochondrial respiratory chain complexes. It locates in the inner membrane of mitochondria and may be involved in mediating the insertion of proteins from the matrix into the inner membrane.

Protocols

Product Specific Protocols
WB protocol for OXA1L antibody 66128-1-IgDownload protocol
IHC protocol for OXA1L antibody 66128-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell

An in vitro system to silence mitochondrial gene expression.

Authors - Luis Daniel Cruz-Zaragoza
humanWB

Cell

Mitochondrial Protein Synthesis Adapts to Influx of Nuclear-Encoded Protein.

Authors - Ricarda Richter-Dennerlein
humanWB

Cell Rep

EMRE Is a Matrix Ca(2+) Sensor that Governs Gatekeeping of the Mitochondrial Ca(2+) Uniporter.

Authors - Horia Vais
mouseWB

Cell Rep

Mitochondrial microproteins link metabolic cues to respiratory chain biogenesis

Authors - Chao Liang