OVGP1 Polyclonal antibody

OVGP1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22324-1-AP

Synonyms

CHIT5, EGP, MUC9, Mucin 9, OGP, OVGP1, Oviduct specific glycoprotein, Oviductal glycoprotein, Oviductin

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Validation Data Gallery

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  WB analysis using 22324-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22324-1-AP (OVGP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse ovary using 22324-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 22324-1-AP (OVGP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: human placenta tissue, mouse ovary tissue, mouse uterus tissue
• Positive IHC detected in: mouse ovary tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

22324-1-AP targets OVGP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: OVGP1 fusion protein Ag17771
• Full Name: oviductal glycoprotein 1, 120kDa
• Calculated Molecular Weight: 678 aa, 75 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC126177
• Gene Symbol: OVGP1
• Gene ID (NCBI): 5016
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

OVGP1 (oviduct-specific glycoprotein, also known as MUC9) is a high molecular-weight glycoprotein thought to be exclusively secreted by the non-ciliated epithelial cells of the fallopian tube (PMID: 12444068). Identified first as an estrogen-induced secretory protein in the oviduct, OVGP1 aids in sperm capacitation, fertilization, and early embryonic development (PMID: 28883914). OVGP1 is the major non-serum protein present in OF (oviduct fluid) and its biological activity differs among species (PMID: 27601270).