OTUD6B Monoclonal antibody

OTUD6B Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse , rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1D1G1

Cat no : 68450-1-Ig

Synonyms

CGI 77, DUBA 5, DUBA5, OTU domain containing 6B, OTUD6B

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Validation Data Gallery

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  WB analysis using 68450-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68450-1-Ig (OTUD6B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: A549 cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68450-1-Ig targets OTUD6B in WB, ELISA applications and shows reactivity with Human, mouse , rat samples.

• Tested Reactivity: Human, mouse , rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: OTUD6B fusion protein Ag33607
• Full Name: OTU domain containing 6B
• Calculated Molecular Weight: 293 aa, 34 kDa
• Observed Molecular Weight: 34-40 kDa
• GenBank Accession Number: BC029760
• Gene Symbol: OTUD6B
• Gene ID (NCBI): 51633
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

OTUD6B, also known as DUBA5, belongs to a DUB subfamily characterized by an ovarian tumor domain (OTU). It's a functional deubiquitinating enzyme, a class of protease that specifically cleaves ubiquitin linkages. OTUD6B function may be connected to growth and proliferation. It has been reported that OTUD6B could significantly suppress HCC cells migration and metastasis. The calculated molecular weight of OTUD6B is 34 kDa, 68450-1-Ig can detect the band around 34 kDa.(PMID:32323143)