OTUB1 Polyclonal antibody

OTUB1 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

1

Reviews (1)

Cat no : 10573-1-AP

Synonyms

Deubiquitinating enzyme OTUB1, hOTU1, HSPC263, OTB1, OTU1, OTUB1, Otubain 1, Ubiquitin thioesterase OTUB1

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Validation Data Gallery

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  WB analysis using 10573-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10573-1-AP (OTUB1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 10573-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10573-1-AP (OTUB1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of U2OS using 10573-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using OTUB1 antibody (10573-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: MCF-7 cells, HepG2 cells, mouse brain tissue, mouse cerebellum tissue, rat brain tissue
• Positive IF detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

10573-1-AP targets OTUB1 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: OTUB1 fusion protein Ag0845
• Full Name: OTU domain, ubiquitin aldehyde binding 1
• Calculated Molecular Weight: 31 kDa and 35 kDa
• Observed Molecular Weight: 31-35 kDa
• GenBank Accession Number: BC007519
• Gene Symbol: OTUB1
• Gene ID (NCBI): 55611
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

OTUB1 belongs to the ovarian tumor domain protease (OTU) subfamily of deubiquitinases, which could block ubiquitination leading to protein stability. OTUB1 mainly prefers k-48 and k-63 ubiquitin chains type, especially in k-48 ubiquitin chain. OTUB1 performs its deubiquitinating function via two distinct manners: the canonical and non-canonical manner. OTUB1 is involved in many critical immune response processes, containing CD8 T cell activation, PD-L1 related immune evasion, and several infectious diseases. OTUB1 plays an important role in the occurrence, proliferation, invasion, and progression of various cancers, such as bladder cancer, breast cancer, prostate cancer, and colorectal cancer (PMID: 34875341, 35296795). OTUB1 has 2 isoforms with molecular weights of 31 and 35 kDa, respectively. With phospho or ubiquitin modification or reaction, the MW of OTUB1 will be increased.

Publications

Species	Application	Title
mouse	WB	iScience; Deacetylation Inhibition Reverses PABPN1-Dependent Muscle Wasting.; Authors - Cyriel S Olie

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Angie (Verified Customer) (04-09-2024)

The antibody detected a band between 25 and 37 kD at dilution of 1:3000 incubated at room temperature for 2 hours. This is followed by secondary antibody donkey-anti-rabbit (Alexa Fluor 800) used at 1:20000 dilution and incubated for 1 hour at room temperature.

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: A549, HEK293

OTUB1 Antibody Western Blot validation (1:3000 dilution) in A549, HEK293 (Cat no:10573-1-AP)