OTOR Polyclonal antibody

OTOR Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 18132-1-AP

Synonyms

FDP, Fibrocyte derived protein, MIAL, MIAL1, OTOR, otoraplin

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Validation Data Gallery

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  WB analysis of Transfected HEK-293 using 18132-1-AP

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 18132-1-AP (OTOR Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 18132-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18132-1-AP (OTOR antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 18132-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18132-1-AP (OTOR antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 18132-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 18132-1-AP (OTOR antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 18132-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 18132-1-AP (OTOR antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: human liver cancer tissue, human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 18132-1-AP is OTOR Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: OTOR fusion protein Ag12714
• Full Name: otoraplin
• Calculated Molecular Weight: 128 aa, 14 kDa
• GenBank Accession Number: BC101688
• Gene Symbol: OTOR
• Gene ID (NCBI): 56914
• RRID: AB_2878505
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.