OSTbeta / SLC51B Polyclonal antibody

OSTbeta / SLC51B Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 21248-1-AP

Synonyms

OST beta, OSTB, OSTbeta, SLC51B

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Validation Data Gallery

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  IHC staining of human small intestine using 21248-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 21248-1-AP (OSTbeta antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human small intestine using 21248-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 21248-1-AP (OSTbeta antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive IHC detected in: human small intestine tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21248-1-AP targets OSTbeta / SLC51B in IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: OSTbeta / SLC51B fusion protein Ag15751
• Full Name: organic solute transporter beta
• Calculated Molecular Weight: 128 aa, 14 kDa
• GenBank Accession Number: BC103842
• Gene Symbol: OSTbeta
• Gene ID (NCBI): 123264
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Organic solute transporter alpha-beta (Ostα-Ostβ) is a heteromeric transporter that has been localized to the basolateral membrane of epithelial cells of the small intestine, kidney and liver, and appears to be essential for bile acid homeostasis. The transporter is composed of a predicted 340-amino acid, 7-transmembrane domain protein (Ostα) and a putative 128-amino acid, single-transmembrane domain polypeptide (Ostβ). Ostα-Ostβ transports bile acids by a facilitated diffusion mechanism; therefore, Ostα-Ostβ can mediate cellular efflux or uptake depending on the substrate's electrochemical gradient. It's reported that Ostα-Ostβ is not only essential for bile acid, but also vital for sterol disposition and enterohepatic circulation(PMID: 21691099).