OSBPL3 Polyclonal antibody

OSBPL3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 12417-1-AP

Synonyms

KIAA0704, ORP 3, ORP3, OSBP related protein 3, OSBP3, OSBPL3

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Validation Data Gallery

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  WB analysis using 12417-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12417-1-AP (OSBPL3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 12417-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12417-1-AP (OSBPL3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 12417-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12417-1-AP (OSBPL3 Antibody) at dilution of 1:50 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 12417-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12417-1-AP (OSBPL3 Antibody) at dilution of 1:50 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HCT 116 cells, K-562 cells, HeLa cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 3 publications below

Product Information

12417-1-AP targets OSBPL3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: OSBPL3 fusion protein Ag3044
• Full Name: oxysterol binding protein-like 3
• Calculated Molecular Weight: 887 aa, 101 kDa
• Observed Molecular Weight: 95-110 kDa
• GenBank Accession Number: BC017731
• Gene Symbol: OSBPL3
• Gene ID (NCBI): 26031
• RRID: AB_2156103
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IHC	BMC Cancer; The expression, immune infiltration, prognosis, and experimental validation of OSBPL family genes in liver cancer; Authors - Kunpeng Tian
human	IHC	Biosci Rep; Identification of hub genes in colorectal cancer based on weighted gene co-expression network analysis.; Authors - Yu Zhang
human	IHC	Biosci Rep; Identification of hub genes in colorectal cancer based on weighted gene co-expression network analysis and clinical data from The Cancer Genome Atlas.; Authors - Yu Zhang