ORC5L Polyclonal antibody

ORC5L Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 11542-1-AP

Synonyms

ORC5, ORC5L, ORC5P, ORC5T

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Validation Data Gallery

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  WB analysis of mouse kidney using 11542-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 11542-1-AP (ORC5L Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse ovary using 11542-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 11542-1-AP (ORC5L antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 11542-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 11542-1-AP (ORC5L antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas cancer using 11542-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11542-1-AP (ORC5L antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HepG2 using 11542-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 11542-1-AP (ORC5L antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse kidney tissue, human kidney tissue, mouse ovary tissue
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 2 publications below

Product Information

11542-1-AP targets ORC5L in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ORC5L fusion protein Ag2133
• Full Name: origin recognition complex, subunit 5-like (yeast)
• Calculated Molecular Weight: 435 aa, 50 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC023652
• Gene Symbol: ORC5L
• Gene ID (NCBI): 5001
• RRID: AB_2267695
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Origin recognition complex (ORC) plays an essential role in the initiation of chromosome DNA replication and transcriptional silencing. ORC binds in an ATP-dependent manner to several well-defined autonomously replicating sequences that serve as the chromosomal replication origins. ORC5L is one of the 6 subunits of ORC.

Publications

Species	Application	Title
human	IHC	Cancer Med; Systemic analysis of the DNA replication regulator origin recognition complex in lung adenocarcinomas identifies prognostic and expression significance; Authors - Min Tang
human	IHC	Oncol Lett; PSMC2, ORC5 and KRTDAP are specific biomarkers for HPV-negative head and neck squamous cell carcinoma.; Authors - Yushen Su