Oligophrenin 1 Polyclonal antibody

Oligophrenin 1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 17445-1-AP

Synonyms

MRX60, oligophrenin 1, OPHN1, OPN1

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Validation Data Gallery

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  WB analysis using 17445-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17445-1-AP (Oligophrenin 1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 17445-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17445-1-AP (Oligophrenin 1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 17445-1-AP

  IP result of anti-Oligophrenin 1 (IP:17445-1-AP, 4ug; Detection:17445-1-AP 1:500) with mouse brain tissue lysate 5200ug.

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  IHC staining of human colon using 17445-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 17445-1-AP (Oligophrenin 1 antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon using 17445-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 17445-1-AP (Oligophrenin 1 antibody at dilution of 1:50 (under 40x lens).

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  IHC staining of human stomach cancer using 17445-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 17445-1-AP (Oligophrenin 1 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human stomach cancer using 17445-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 17445-1-AP (Oligophrenin 1 antibody at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse skeletal muscle tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human colon tissue, human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

17445-1-AP targets Oligophrenin 1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Oligophrenin 1 fusion protein Ag11538
• Full Name: oligophrenin 1
• Calculated Molecular Weight: 802 aa, 92 kDa
• Observed Molecular Weight: 91 kDa
• GenBank Accession Number: BC059393
• Gene Symbol: OPHN1
• Gene ID (NCBI): 4983
• RRID: AB_2158304
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The OPHN1 gene encodes a Rho-GTPase activating protein (RhoGAP), and mutations in OPHN1 are responsible for non-specific X-linked mental retardation (NSMR). OPHN1 is highly expressed in the brain and present both pre- and postsynaptically in neurons.

Publications

Species	Application	Title
mouse	WB	Cell Rep; Error-prone protein synthesis recapitulates early symptoms of Alzheimer disease in aging mice; Authors - Margarita Brilkova