Oligophrenin 1 Polyclonal antibody

Oligophrenin 1 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 11076-1-AP

Synonyms

MRX60, oligophrenin 1, OPHN1, OPN1

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Validation Data Gallery

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  WB analysis of HeLa using 11076-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11076-1-AP (Oligophrenin 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 11076-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11076-1-AP (Oligophrenin 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 11076-1-AP

  IP result of anti-Oligophrenin 1 (IP:11076-1-AP, 4ug; Detection:11076-1-AP 1:300) with mouse brain tissue lysate 4000 ug.

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  IHC staining of human cerebellum using 11076-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 11076-1-AP (Oligophrenin 1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cerebellum using 11076-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 11076-1-AP (Oligophrenin 1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human retinoblastoma using 11076-1-AP

  Immunohistochemical analysis of paraffin-embedded human retinoblastoma tissue slide using 11076-1-AP (Oligophrenin 1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human retinoblastoma using 11076-1-AP

  Immunohistochemical analysis of paraffin-embedded human retinoblastoma tissue slide using 11076-1-AP (Oligophrenin 1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human retinoblastoma using 11076-1-AP

  Immunohistochemical analysis of paraffin-embedded human retinoblastoma tissue slide using 11076-1-AP (Oligophrenin 1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human retinoblastoma using 11076-1-AP

  Immunohistochemical analysis of paraffin-embedded human retinoblastoma tissue slide using 11076-1-AP (Oligophrenin 1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 11076-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 11076-1-AP (Oligophrenin 1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, mouse brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human cerebellum tissue, human retinoblastoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 2 publications below

Product Information

11076-1-AP targets Oligophrenin 1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Oligophrenin 1 fusion protein Ag1536
• Full Name: oligophrenin 1
• Calculated Molecular Weight: 802 aa, 92 kDa
• Observed Molecular Weight: 92 kDa
• GenBank Accession Number: BC059393
• Gene Symbol: OPHN1
• Gene ID (NCBI): 4983
• RRID: AB_2158306
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The OPHN1 gene encodes a Rho-GTPase activating protein (RhoGAP), and mutations in OPHN1 are responsible for non-specific X-linked mental retardation (NSMR). OPHN1 is highly expressed in the brain and present both pre- and postsynaptically in neurons.

Publications

Species	Application	Title
human	IHC	Ann Surg Oncol; Additive lymph node dissection may be necessary in minute submucosal cancer of the stomach after endoscopic resection.; Authors - Kim Jie-Hyun JH
human	WB	PLoS One; Oligophrenin-1 (OPHN1), a gene involved in X-linked intellectual disability, undergoes RNA editing and alternative splicing during human brain development.; Authors - Sabina Barresi
mouse	WB,IHC	Gene Expr Patterns; Oligophrenin-1 (Ophn1) is expressed in mouse retinal vessels.; Authors - Sel Saadettin S
mouse	WB	Neurosci Lett; Alpha lipoic acid treatment in late middle age improves cognitive function: Proteomic analysis of the protective mechanisms in the hippocampus; Authors - Jian Zhang