OGN Monoclonal antibody

OGN Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2A5F11

Cat no : 66382-1-Ig

Synonyms

Mimecan, OG, OGN, OIF, osteoglycin, Osteoinductive factor, SLRR3A



Tested Applications

Positive WB detected inhuman artery tissue, pig artery tissue
Positive IHC detected inhuman liver tissue, human lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66382-1-Ig targets OGN in WB, IHC, ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen OGN fusion protein Ag3484
Full Name osteoglycin
Calculated Molecular Weight 298 aa, 34 kDa
Observed Molecular Weight 30-40 kDa
GenBank Accession NumberBC037273
Gene Symbol OGN
Gene ID (NCBI) 4969
RRIDAB_2881758
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

This gene encodes a member of the small leucine-rich proteoglycan (SLRP) family of proteins. The encoded protein induces ectopic bone formation in conjunction with transforming growth factor beta and may regulate osteoblast differentiation. High expression of the encoded protein may be associated with elevated heart left ventricular mass. Alternative splicing results in multiple transcript variants which forms proteins with different molecular weight.

Protocols

Product Specific Protocols
WB protocol for OGN antibody 66382-1-IgDownload protocol
IHC protocol for OGN antibody 66382-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols