ODF3 Polyclonal antibody

ODF3 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 25097-1-AP

Synonyms

hSHIPPO, ODF3, Outer dense fiber protein 3, SHIPPO1, Sperm tail protein SHIPPO 1, TISP50

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Validation Data Gallery

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  WB analysis of rat testis using 25097-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 25097-1-AP (ODF3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 25097-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25097-1-AP (ODF3 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 25097-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25097-1-AP (ODF3 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: rat testis tissue
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25097-1-AP is ODF3 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ODF3 fusion protein Ag18734
• Full Name: outer dense fiber of sperm tails 3
• Calculated Molecular Weight: 254 aa, 28 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC126248
• Gene Symbol: ODF3
• Gene ID (NCBI): 113746
• RRID: AB_2879894
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.