OATP14 Polyclonal antibody

OATP14 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 17163-1-AP

Synonyms

OAT RP 5, OATP 14, OATP F, OATP1, OATP14, OATP1C1, OATPF, OATPRP5, Organic anion transporter F, SLC21A14, SLCO1C1

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Validation Data Gallery

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  WB analysis of PC-3 using 17163-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 17163-1-AP (OATP14 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 17163-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17163-1-AP (OATP14 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 17163-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17163-1-AP (OATP14 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 17163-1-AP

  IP result of anti-OATP14 (IP:17163-1-AP, 4ug; Detection:17163-1-AP 1:800) with mouse brain tissue lysate 4000ug.

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Tested Applications

• Positive WB detected in: PC-3 cells, mouse brain tissue, mouse kidney tissue
• Positive IP detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

17163-1-AP targets OATP14 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: OATP14 fusion protein Ag10573
• Full Name: solute carrier organic anion transporter family, member 1C1
• Calculated Molecular Weight: 712 aa, 79 kDa
• Observed Molecular Weight: 62-70 kDa
• GenBank Accession Number: BC022461
• Gene Symbol: SLCO1C1
• Gene ID (NCBI): 53919
• RRID: AB_2189706
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Toxicol Sci; Hepatic, metabolic and toxicity evaluation of repeated oral administration of SnS2 nanoflowers in mice.; Authors - Disi Bai
human	WB	Toxicology; BDE209-promoted Dio2 degradation in H4 glioma cells through the autophagy pathway, resulting in hypothyroidism and leading to neurotoxicity; Authors - Min Liu