OAS1 Recombinant antibody

OAS1 Recombinant Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2H4

Cat no : 82883-1-RR

Synonyms

(2 5)oligo(A) synthase 1, 2 5 oligoadenylate synthase 1, 2 5A synthase 1, E18/E16, IFI 4, OAS1, OIAS, OIASI, p46/p42 OAS

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Validation Data Gallery

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  WB analysis using 82883-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82883-1-RR (OAS1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 82883-1-RR

  A549 cells were subjected to SDS PAGE followed by western blot with 82883-1-RR (OAS1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse liver using 82883-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 82883-1-RR (OAS1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 82883-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 82883-1-RR (OAS1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 82883-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 82883-1-RR (OAS1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 82883-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 82883-1-RR (OAS1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, A549 cells
• Positive IHC detected in: mouse liver tissue, mouse spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82883-1-RR targets OAS1 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

• Tested Reactivity: Human, Mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: OAS1 fusion protein Ag6793
• Full Name: 2',5'-oligoadenylate synthetase 1, 40/46kDa
• Calculated Molecular Weight: 46 kDa
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: BC000562
• Gene Symbol: OAS1
• Gene ID (NCBI): 4938
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The 2-prime,5-prime oligoadenylate synthetases (OASs), such as OAS1, are interferon-induced proteins characterized by their capacity to catalyze the synthesis of 2-prime,5-prime oligomers of adenosine (2-5As). OAS1 (type I enzymes) has some isoforms with the MW of 40, 42, 44, 46, and 48 kDa (PMID:12590567, 19383565). OAS1 is a strong candidate for determining susceptibility or resistance to viral infections(PMID:15732009).