NeutraControl Thrombopoietin Monoclonal antibody

NeutraControl Thrombopoietin Monoclonal Antibody for ELISA, Non-Neutralization

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

ELISA, Non-Neutralization

Conjugate

Unconjugated

CloneNo.

1H3G12

Cat no : 69528-1-Ig

Synonyms

C mpl ligand, MGDF, MKCSF, ML, MPLLG, THPO, thrombopoietin, TPO

---

Validation Data Gallery

• 

  Non-Neutralization experiment of TF-1 using 69528-1-Ig

  Indirect ELISA was carried out by coating recombinant Human Thrombopoietin (Cat.NO. HZ-1248) at 70 ng/well followed by blocking and adding serial diluted Thrombopoietin antibody 69028-1-Ig and 69528-1-Ig respectively. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm.

• 

  ELISA experiment of Recombinant protein using 69528-1-Ig

  Recombinant human Thrombopoietin (Cat.NO. HZ-1248) stimulates proliferation of TF-1 cell line (human erythroleukemic cell line) in a dose-dependent manner (blue curve, refer to bottom X-left Y). The activity of human Thrombopoietin (30 ng/mL HZ-1248) is neutralized by mouse anti-human Thrombopoietin monoclonal antibody 69028-1-Ig at serial dose (red curve, refer to top X-right Y). The ND50 is typically 0.1-0.5μg/mL.The NeutraControl mouse anti-human Thrombopoietin antibody 69528-1-Ig could recognize human Thrombopoietin but could not neutralize human Thrombopoietin acitivity in the range of 0.00122-20 μg/mL (green curve, refer to top X-right Y axis).

---

Product Information

69528-1-Ig targets Thrombopoietin in ELISA, Non-Neutralization applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: human Humankine Thrombopoietin protein HZ-1248
• Full Name: thrombopoietin
• Gene Symbol: THPO
• Gene ID (NCBI): 7066
• Conjugate: Unconjugated
• Form: Lyophilized Powder
• Purification Method: Protein A purification
• Storage Buffer: Sterile PBS.
• Endotoxin: <0.1 EU/μg
• Reconstitution: This product was lyophilized from a 0.2 μm filtered solution in PBS. Reconstitute at 1.0 mg/mL in sterile H2O before use.
• Stability and Storage: Lyophilized antibodies are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.

Background Information

Thrombopoietin (TPO) is a primary regulator of megakaryocyte development and platelet production in mammals. Human TPO constitutively circulates and maintains thrombopoiesis through interaction with its cognate receptor, myeloproliferative leukemia protein(MPL). TPO also plays an important role in the maintenance and regulation of hematopoietic stem cells (HSCs).

This antibody is a neutralizing control antibody for THPO, the immunogen is the same as Neutrakine 69028-1-Ig but could not neutralize human TPO.