NUPR1 Polyclonal antibody

• Featured Product
• KD/KO Validated

NUPR1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 15056-1-AP

Synonyms

Candidate of metastasis 1, COM1, nuclear protein 1, NUPR1, P8, Protein p8

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Validation Data Gallery

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  WB analysis of L02 using 15056-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 15056-1-AP (NUPR1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 15056-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15056-1-AP (NUPR1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 15056-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15056-1-AP (NUPR1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 15056-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15056-1-AP (NUPR1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 15056-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15056-1-AP (NUPR1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 15056-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15056-1-AP (NUPR1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 15056-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15056-1-AP (NUPR1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: L02 cells
• Positive IHC detected in: human colon cancer tissue, human prostate cancer tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:300-1:600
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 7 publications below
• IHC: See 5 publications below
• IF: See 1 publications below
• CoIP: See 2 publications below

Product Information

15056-1-AP targets NUPR1 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NUPR1 fusion protein Ag7039
• Full Name: nuclear protein 1
• Calculated Molecular Weight: 8 kDa
• Observed Molecular Weight: 8 kDa, 17 kDa, 38 kDa
• GenBank Accession Number: BC002434
• Gene Symbol: NUPR1
• Gene ID (NCBI): 26471
• RRID: AB_2878104
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NUPR1, also named as COM1 and P8, is a small nuclear protein that is responsive to various stress stimuli. NUPR1 can be induced by hypoxia, oxidative stress, while the expression is low in physiological condition. It is associated with chemoresistance in multiple malignancies. The predicted MW of NUPR1 is 8 kDa and we also detectet bands around 17 and 38 kDa, which are similar to the papers(PMID: 29130426, 27336713, 28694771, 22961798). NUPR1 was also found in a complex with ANXA2, TRIM21, YBX1, S100A9, HSPA8, HSPA9, HSPA4, HSPA5, and ESR1, suggesting that these proteins might play a role in its functional regulation (PMID: 33542201), and it can also form a complex with PADI4 and plays crucial functions in modulating DNA-repair, favoring metastasis, or facilitating citrullination of other proteins (PMID: 36858171).

Publications

Species	Application	Title
human,mouse	WB,IHC,IF,CoIP	BMC Med; NUPR1 contributes to radiation resistance by maintaining ROS homeostasis via AhR/CYP signal axis in hepatocellular carcinoma; Authors - Yizhi Zhan ; KD Validated
human	WB	J Inorg Biochem; Chronic nickel exposure alters extracellular vesicles to mediate cancer progression via sustained NUPR1 expression; Authors - Shan Liu ; KD Validated
human	WB	PLoS One; Adenomatous Polyposis Coli loss controls cell cycle regulators and response to paclitaxel in MDA-MB-157 metaplastic breast cancer cells.; Authors - Emily M Astarita
human,mouse	IHC,CoIP	Cell Death Discov; The interplay of transcriptional coregulator NUPR1 with SREBP1 promotes hepatocellular carcinoma progression via upregulation of lipogenesis; Authors - Yongjia Wang ; KO Validated
human	WB,IHC	Cancer Med; NUPR1 imparts oncogenic potential in bladder cancer; Authors - Lifeng Zhang ; KD Validated
human	WB	PPAR Res; Nuclear Protein 1 Expression Is Associated with PPARG in Bladder Transitional Cell Carcinoma; Authors - Chao Lu