NUP85 Polyclonal antibody

NUP85 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 30158-1-AP

Synonyms

85 kDa nucleoporin, FLJ12549, FROUNT, nucleoporin 85kDa, Nucleoporin Nup75, Nucleoporin Nup85, Nup75, NUP85

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Validation Data Gallery

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  WB analysis using 30158-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30158-1-AP (NUP85 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 30158-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30158-1-AP (Pericentrin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse liver tissue, rat liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30158-1-AP targets NUP85 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NUP85 fusion protein Ag33004
• Full Name: nucleoporin 85kDa
• Calculated Molecular Weight: 75 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC000697
• Gene Symbol: NUP85
• Gene ID (NCBI): 79902
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nucleoporin 85 (Nup85) (also known as FROUNT) is a protein that in humans is encoded by the NUP85 gene. This gene encodes a protein component of the Nup107-160 subunit of the nuclear pore complex. Nuclear pore complexes are embedded in the nuclear envelope and promote bidirectional transport of macromolecules between the cytoplasm and nucleus. The encoded protein can also bind to the C-terminus of chemokine (C-C motif) receptor 2 (CCR2) and promote chemotaxis of monocytes, thereby participating in the inflammatory response.