NUF2 Polyclonal antibody

• Featured Product
• KD/KO Validated

NUF2 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

5

Reviews (1)

Cat no : 15731-1-AP

Synonyms

CDCA1, hNuf2, hNuf2R, hsNuf2, Kinetochore protein Nuf2, NUF2, NUF2R

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Validation Data Gallery

• 

  WB analysis using 15731-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15731-1-AP (NUF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 15731-1-AP

  WB result of NUF2 antibody (15731-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NUF2 transfected HepG2 cells.

• 

  WB analysis of human skeletal muscle using 15731-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15731-1-AP (NUF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15731-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15731-1-AP (NUF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 15731-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 15731-1-AP (NUF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 15731-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 15731-1-AP (NUF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 15731-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 15731-1-AP (NUF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 15731-1-AP

  IP result of anti-NUF2 (IP:15731-1-AP, 3ug; Detection:15731-1-AP 1:500) with HEK-293 cells lysate 1760ug.

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Tested Applications

• Positive WB detected in: HEK-293 cells, HepG2 cells, HeLa cells, human liver tissue, human testis tissue, PC-3 cells, human skeletal muscle tissue
• Positive IP detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 1 publications below

Product Information

15731-1-AP targets NUF2 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NUF2 fusion protein Ag8479
• Full Name: NUF2, NDC80 kinetochore complex component, homolog (S. cerevisiae)
• Calculated Molecular Weight: 464 aa, 54 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC008489
• Gene Symbol: NUF2
• Gene ID (NCBI): 83540
• RRID: AB_2154586
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NUF2 (NUF2, Ndc80 kinetochore complex component) plays an important role in kinetochore-microtubule attachment. NUF2 was reported to be overexpressed in various kinds of carcinomas including lung cancer, cholangiocellular cancer, urinary bladder cancer, renal cell cancer, colorectal cancer, gastric cancer, ovarian cancer and serous adenocarcinoma. Elevated expression of NUF2 was associated with poorer prognosis of non-small cell lung cancer patients (PMID: 17079454). Knockdown of NUF2 significantly caused cell growth delay and increased apoptosis in cell lines of non-small cell lung cancer, colorectal cancer, gastric cancer and ovarian cancer.

Publications

Species	Application	Title
human	WB	Cell Death Dis; LncRNA CDKN2B-AS1 stabilized by IGF2BP3 drives the malignancy of renal clear cell carcinoma through epigenetically activating NUF2 transcription.; Authors - Xina Xie
human	WB,IHC	Int J Biol Sci; NUF2 Drives Clear Cell Renal Cell Carcinoma by Activating HMGA2 Transcription through KDM2A-mediated H3K36me2 Demethylation; Authors - Jiatian Lin ; KD Validated
human,mouse	WB	Front Oncol; NUF2 overexpression contributes to epithelial ovarian cancer progression via ERBB3-mediated PI3K-AKT and MAPK signaling axes; Authors - Ruobing Leng ; KD Validated
human		Nat Commun; LUBAC controls chromosome alignment by targeting CENP-E to attached kinetochores.; Authors - Min Wu
human	WB	Sci Rep; Label-free Quantitative Analysis of Protein Expression Alterations in miR-26a-Knockout HeLa Cells using SWATH-MS Technology.; Authors - Hexiao Shen

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Katie (Verified Customer) (05-23-2022)

Works well for western

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: T cells