CoraLite®594-conjugated NT5E,CD73 Monoclonal antibody

NT5E,CD73 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1G5G8

Cat no : CL594-67789

Synonyms

5 NT, 5 nucleotidase, 5' nucleotidase, ecto (CD73), CD73, E5NT, Ecto 5 nucleotidase, eN, eNT, NT, NT5, NT5E, NT5E,CD73, NTE

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Validation Data Gallery

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  IF Staining of human tonsillitis using CL594-67789

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 NT5E,CD73 antibody (CL594-67789, Clone: 1G5G8 ) at dilution of 1:200.

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Tested Applications

• Positive IF detected in: human tonsillitis tissue

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67789 targets NT5E,CD73 in IF applications and shows reactivity with Human, Rat samples.

• Tested Reactivity: Human, Rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: NT5E,CD73 fusion protein Ag30616
• Full Name: 5'-nucleotidase, ecto (CD73)
• Calculated Molecular Weight: 29 kDa, 63 kDa
• Observed Molecular Weight: 60-70 kDa
• GenBank Accession Number: BC015940
• Gene Symbol: NT5E
• Gene ID (NCBI): 4907
• RRID: AB_2920183
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD73, also known as ecto-5'-nucleotidase (5'-NT), is a 70-kDa, glycosyl-phosphatidylinositol-linked membrane-bound glycoprotein found in most tissues (PMID: 18404475; 20179192). CD73 is an ectoenzyme that catalyzes the dephosphorylation of AMP and other nucleoside monophosphates (PMID: 9553767). In the human immune system, CD73 is expressed on subsets of T and B cells, on germinal center follicular dendritic cells, and on thymic medullary reticular fibroblasts and epithelial cells (PMID: 2137649; 9553767). CD73 is highly expressed in many human solid tumors and is closely involved in cancer progression (PMID: 20179192).