NT5C3 Polyclonal antibody

NT5C3 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 11393-1-AP

Synonyms

5' nucleotidase, cytosolic III, cN III, Cytosolic 5 nucleotidase 3, Cytosolic 5 nucleotidase III, NT5C3, p36, P5N 1, P5'N 1, P5N1, PN I, PSN1, Pyrimidine 5 nucleotidase 1, UMPH, UMPH1

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Validation Data Gallery

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  WB analysis of human skeletal muscle using 11393-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11393-1-AP (NT5C3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse placenta using 11393-1-AP

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 11393-1-AP (NT5C3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 11393-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 11393-1-AP (NT5C3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 11393-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11393-1-AP (NT5C3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse kidney using 11393-1-AP

  IP result of anti-NT5C3 (IP:11393-1-AP, 4ug; Detection:11393-1-AP 1:500) with mouse kidney tissue lysate 3000 ug.

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  IHC staining of human colon cancer using 11393-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11393-1-AP (NT5C3 antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: human skeletal muscle tissue, mouse kidney tissue, mouse placenta tissue
• Positive IP detected in: mouse kidney tissue
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

11393-1-AP targets NT5C3 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NT5C3 fusion protein Ag1949
• Full Name: 5'-nucleotidase, cytosolic III
• Calculated Molecular Weight: 297 aa, 34 kDa
• Observed Molecular Weight: 33-38 kDa
• GenBank Accession Number: BC015856
• Gene Symbol: NT5C3
• Gene ID (NCBI): 51251
• RRID: AB_2267361
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Nat Biotechnol; Quantitative sequencing using BID-seq uncovers abundant pseudouridines in mammalian mRNA at base resolution; Authors - Qing Dai