NSUN6 Polyclonal antibody

• Featured Product
• KD/KO Validated

NSUN6 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, RIP, IHC, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 17240-1-AP

Synonyms

4933414E04Rik, NOPD1, NSUN6

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Validation Data Gallery

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  WB analysis using 17240-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17240-1-AP (NSUN6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17240-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17240-1-AP (NSUN6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 17240-1-AP

  WB result of NSUN6 antibody (17240-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NSUN6 transfected HepG2 cells.

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  IHC staining of human colon cancer using 17240-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17240-1-AP (NSUN6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 17240-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17240-1-AP (NSUN6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Neuro-2a cells, HEK-293 cells, HepG2 cells, mouse brain
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 6 publications below
• WB: See 6 publications below
• IHC: See 3 publications below
• IF: See 1 publications below
• RIP: See 1 publications below

Product Information

17240-1-AP targets NSUN6 in WB, IF, RIP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NSUN6 fusion protein Ag11084
• Full Name: NOL1/NOP2/Sun domain family, member 6
• Calculated Molecular Weight: 469 aa, 52 kDa
• Observed Molecular Weight: 52-55 kDa
• GenBank Accession Number: BC035778
• Gene Symbol: NSUN6
• Gene ID (NCBI): 221078
• RRID: AB_2878367
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NSUN6 is an RNA methyltransferase that catalyzes the methylation at position cytosine C72 in some tRNAs. NSUN6 has been reported to localize to the cytoplasm and largely colocalize with Golgi apparatus and pericentriolar matrix, indicating that NSUN6 modifies tRNAs in a late step in their biogenesis. NSUN6 may also play a major role in bone metastasis and could be a valuable therapeutic target for bone metastasis.

Publications

Species	Application	Title
human	WB	Nucleic Acids Res; Sequence- and structure-specific cytosine-5 mRNA methylation by NSUN6.; Authors - Tommaso Selmi ; KO Validated
human	WB	PLoS Biol; Cytosine-5 RNA methylation links protein synthesis to cell metabolism.; Authors - Nikoletta A Gkatza
human	WB	Mol Syst Biol; CIGAR-seq, a CRISPR/Cas-based method for unbiased screening of novel mRNA modification regulators.; Authors - Liang Fang ; KD Validated
human	WB	Nat Struct Mol Biol; Genome-wide identification of mRNA 5-methylcytosine in mammals.; Authors - Tao Huang ; KD Validated
human,mouse	WB,IHC,RIP	Exp Ther Med; Loss of NSUN6 inhibits osteosarcoma progression by downregulating EEF1A2 expression and activation of Akt/mTOR signaling pathway via m5C methylation; Authors - Sang Hu ; KD Validated
human	IHC,IF	J Cell Mol Med; RNA m5C methylation orchestrates BLCA progression via macrophage reprogramming; Authors - Dali Yan ; KO Validated