NSF Monoclonal antibody

• Featured Product
• KD/KO Validated

NSF Monoclonal Antibody for WB, IF, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Pig, Rabbit, Rat, Mouse, Chicken

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2B6H6

Cat no : 68061-1-Ig

Synonyms

NEM sensitive fusion protein, NSF, SKD2, Vesicle fusing ATPase, Vesicular fusion protein NSF

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Validation Data Gallery

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  WB analysis using 68061-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68061-1-Ig (NSF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-12 using 68061-1-Ig

  PC-12 cells were subjected to SDS PAGE followed by western blot with 68061-1-Ig (NSF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 68061-1-Ig

  WB result of NSF antibody (68061-1-Ig; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NSF transfected HEK-293 cells.

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  WB analysis of SH-SY5Y using 68061-1-Ig

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 68061-1-Ig (NSF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse brain using 68061-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using NSF antibody (68061-1-Ig, Clone: 2B6H6 ) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: pig brain tissue, SH-SY5Y cells, PC-12 cells, HEK-293 cells, Rabbit brain tissue, Rat brain tissue, Mouse brain tissue, Chicken brain tissue
• Positive IF detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF): IF : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68061-1-Ig targets NSF in WB, IF, ELISA applications and shows reactivity with Human, Pig, Rabbit, Rat, Mouse, Chicken samples.

• Tested Reactivity: Human, Pig, Rabbit, Rat, Mouse, Chicken
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: NSF fusion protein Ag15422
• Full Name: N-ethylmaleimide-sensitive factor
• Calculated Molecular Weight: 744 aa, 83 kDa
• Observed Molecular Weight: 70-83 kDa
• GenBank Accession Number: BC030613
• Gene Symbol: NSF
• Gene ID (NCBI): 4905
• RRID: AB_2918802
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.